Junfeng Wang's Lab

Welcome to Biomolecular NMR laboratory

This is the homepage of Professor Junfeng Wang's group at Chinese high magnetic field laboratory . We focus on studying the three dimensional structures and functions of disease-related membrane proteins using NMR technique.

机时预约平台：http://wanyfs11.x7.fjjsp.com/

About Us

Our Research Team

Junfeng Wang, Ph. D

Dr. Junfeng Wang, director of the magnetic resonance division and the director of the biomolecular NMR program. He received his PhD degree from National High Magnetic Field Laboratory/Florida State University in 2001. Before joining High Magnetic Field Laboratory as a researcher of Chinese Academy of Sciences, he conducted postdoctoral researches at University of Georgia and Harvard Medical School.

Phone:+86-551-65591672

Email:junfeng@hmfl.ac.cn

Research

Many important biological functions, such as the cross-membrane nutrients transport, neural signal transduction, cell-cell interactions are implemented by membrane proteins, which count for 30% of the protein genomes. Mutations and dysfunctions of membrane proteins and membrane-associated proteins result in various diseases, and over 60% of current drug targets are membrane proteins. The ongoing researches in the lab focus on membrane protein structure determination and protein-lipid interactions, with the goal of providing structural and dynamical insights on cross-membrane signaling and other protein-membrane recognition events. The primary tool we are using is solution NMR spectroscopy, which is ideal for determining protein structure, characterizing protein dynamics and inter-molecular interactions in solution.

Publications

1.Ma, X. , Wang, L. , Huang, D. , Li, Y. , Yang, D. , & Li, T. , et al. (2017). Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. Nature Communications, 8(1), 1506.

2.Ma, K., H. Zhao, X. Zheng, H. Sun, L. Hu, L. Zhu, Y. Shen, T. Luo, H. Dai and J. Wang (2017). "NMR studies of the interactions between AMB-1 Mms6 protein and magnetosome Fe3O4 nanoparticles." Journal of Materials Chemistry B 5(16): 2888-2895

3.Guolin Ma#, Ming Wei#, Lian He#, Chongxu Liu#, Bo Wu, Shenyuan L Zhang, Ji Jing，Xiaowen Liang，Alessandro Senes，Peng Tan，Yunchen Bi，Ling Zhong，Hongjiang Si，Yuequan Shen，Minyong Li，Mi-Sun Lee，Weibin Zhou，Junfeng Wang*，Youjun Wang*，Yubin Zhou*, (2015) Inside-out Ca2+ signalling prompted by STIM1 conformational switch. Nature Comm. 6:7826.

4.Yue Yuan#，Shuchao Ge#，Hongbin Sun#，Xuejiao Dong，Hongxin Zhao，Linna An，Jia Zhang，Junfeng Wang*，Bing Hu*，Gaolin Liang*, (2015), Intracellular Self-Assembly and Disassembly of 19 F Nanoparticles Confer Respective “Off” and “On” 19 F NMR/MRI Signals for Legumain Activity Detection in Zebrafish. ACS Nano 9(5):5117-5124.

5.Yue Yuan#, Hongbin Sun#, Shuchao Ge#, Mengjing Wang, Hongxin Zhao, Lin Wang, Linna An, Jia Zhang,Huafeng Zhang, Bing Hu*, Junfeng Wang*, Gaolin Liang*. (2015)，Controlled Intracellular Self-Assembly and Disassembly of 19F Nanoparticles for MR Imaging of Caspase 3/7 in Zebrafish. ACS Nano, 9(1):761-768.

6.Fazhan Shi, Qi Zhang, Pengfei Wang, Hongbin Sun, Jiarong Wang, Xing Rong, Ming Chen, Chenyong Ju, Friedmann Reinhard, Hongwei Chen, Jorg Wrachtrup, Junfeng Wang, Jiangfeng Du*, Single-protein spin resonance spectroscopy under ambient conditions. Science, 347(6226):1135-8.

7. Meng Wang, Xin-Ping Wu, Sujuan Zheng, Li Zhao, Lei Li, Li Shen, Yuxian Gao, Nianhua Xue, Xuefeng Guo, Weixin Huang, Zhehong Gan, Frédéric Blanc, Zhiwu Yu, Xiaokang Ke, Weiping Ding, Xue-Qing Gong*, Clare P. Grey, and Luming Peng*(2015), Identification of different oxygen species in oxide nanostructures with 17O solid-state NMR spectroscopy, Science Advances, DOI: 10.1126/sciadv.1400133.

8.Shunrun Chen, Zhiwu Yu#, Qi Zhang, Junping Wang, Teng Zhang, Junfeng Wang*, (2015), Reducing the interfacial reaction between borosilicate sealant andyttria-stabilized zirconia electrolyte by addition of HfO2, Journal of the Eurooean Ceramic Society. 35, 2427–2431.

9.Shi X#, Bi Y#, Yang W#, Guo X, Jiang Y, Wan C, Li L, Bai Y, Guo J, Wang Y, Chen X, Wu B, Sun H, Liu W, Wang J*, Xu C*. (2013) Ca2+ regulates T cell receptor activation by modulating phospholipid electrostatic property. Nature 493(7430):111-5. (#Co-first authors, *co-corresponding authors)

10.J. Wang, R. Pielak, M. McClintock and J. Chou, （2009）"Solution Structure and Functional Analysis of the Influenza B Proton Channel", (featured story) Nature Struct. Mol. Biol. 16(12):1267-1272.

Members

News

2015-09-24 Report

Dr.Jean-Paul Amoureux(University of lille-1)

Presentation Title:New methods for the detection of 14N nuclei in solid state NMR

When: 9:00 on Sep 24th, 2015

Where:322 CHMFL

Host: Dr. Junfeng Wang

2015-06-03 Report

Dr. Haribabu Arthanari (Harvard Medical School)

Presentation Title: Therapeutic Targeting of Protein-Protein Interactions: Deciphering the protein landscape by NMR Spectroscopy

When: 15:00 on June 3rd, 2015

Where: 201 CHMFL

Host: Dr. Junfeng Wang

Collect from Website Template

JUNFENG WANG'S LAB

Welcome to Biomolecular NMR laboratory

机时预约平台：http://wanyfs11.x7.fjjsp.com/

About Us

Our Research Team

Junfeng Wang, Ph. D

Phone:+86-551-65591672

Email:junfeng@hmfl.ac.cn

Research

Publications

1.Ma, X. , Wang, L. , Huang, D. , Li, Y. , Yang, D. , & Li, T. , et al. (2017). Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. Nature Communications, 8(1), 1506.

2.Ma, K., H. Zhao, X. Zheng, H. Sun, L. Hu, L. Zhu, Y. Shen, T. Luo, H. Dai and J. Wang (2017). "NMR studies of the interactions between AMB-1 Mms6 protein and magnetosome Fe3O4 nanoparticles." Journal of Materials Chemistry B 5(16): 2888-2895

6.Fazhan Shi, Qi Zhang, Pengfei Wang, Hongbin Sun, Jiarong Wang, Xing Rong, Ming Chen, Chenyong Ju, Friedmann Reinhard, Hongwei Chen, Jorg Wrachtrup, Junfeng Wang, Jiangfeng Du*, Single-protein spin resonance spectroscopy under ambient conditions. Science, 347(6226):1135-8.

8.Shunrun Chen, Zhiwu Yu#, Qi Zhang, Junping Wang, Teng Zhang, Junfeng Wang*, (2015), Reducing the interfacial reaction between borosilicate sealant andyttria-stabilized zirconia electrolyte by addition of HfO2, Journal of the Eurooean Ceramic Society. 35, 2427–2431.

9.Shi X#, Bi Y#, Yang W#, Guo X, Jiang Y, Wan C, Li L, Bai Y, Guo J, Wang Y, Chen X, Wu B, Sun H, Liu W, Wang J*, Xu C*. (2013) Ca2+ regulates T cell receptor activation by modulating phospholipid electrostatic property. Nature 493(7430):111-5. (#Co-first authors, *co-corresponding authors)

10.J. Wang, R. Pielak, M. McClintock and J. Chou, （2009）"Solution Structure and Functional Analysis of the Influenza B Proton Channel", (featured story) Nature Struct. Mol. Biol. 16(12):1267-1272.

Members

Professors

Hongbin Sun hbsun@hmfl.ac.cn

Associate professors

Yujuan Wang yjwang@hmfl.ac.cn

Zhiwu Yu zhiwuyu@hmfl.ac.cn

Assistant researchers

Bo Wu bowu@hmfl.ac.cn

Wenping Mao mao@hmfl.ac.cn

Jiarong Wang jiarongwang@hmfl.ac.cn

Jing Yang yj91@hmfl.ac.cn

Yunyan Li yyli@hmfl.ac.cn

Graduate student

Kun Ma makun@hmfl.ac.cn

Juanjuan Liu liujuan@hmfl.ac.cn

Zhe Zhang kids@mail.ustc.ac.cn

Hongxin Zhao zhx@hmfl.ac.cn

Chongxu Liu chongxu@hmfl.ac.cn

Chun Ye cye927@mail.ustc.edu.cn

Dongbei Li 651117671@qq.com

Shuai Xu qtxsxs@163.com

Liang Ge ahyz108@163.com

Maosen Ruan 1406172513@qq.com

Postdoctor

Lei Zhu zhulei@hmfl.ac.cn

News

6.Fazhan Shi, Qi Zhang, Pengfei Wang, Hongbin Sun, Jiarong Wang, Xing Rong, Ming Chen, Chenyong Ju, Friedmann Reinhard, Hongwei Chen, Jorg Wrachtrup, Junfeng Wang, Jiangfeng Du, Single-protein spin resonance spectroscopy under ambient conditions. Science,* 347(6226):1135-8.

8.Shunrun Chen, Zhiwu Yu#, Qi Zhang, Junping Wang, Teng Zhang, Junfeng Wang, (2015), Reducing the interfacial reaction between borosilicate sealant andyttria-stabilized zirconia electrolyte by addition of HfO2, Journal of the Eurooean Ceramic Society*. 35, 2427–2431.

9.Shi X#, Bi Y#, Yang W#, Guo X, Jiang Y, Wan C, Li L, Bai Y, Guo J, Wang Y, Chen X, Wu B, Sun H, Liu W, Wang J, Xu C. (2013) Ca2+ regulates T cell receptor activation by modulating phospholipid electrostatic property. Nature 493(7430):111-5. (#Co-first authors, *co-corresponding authors)