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Welcome to Biomolecular NMR laboratory

This is the homepage of Professor Junfeng Wang's group at Chinese high magnetic field laboratory . We focus on studying the three dimensional structures and functions of disease-related membrane proteins  using NMR technique.


About Us

Junfeng Wang, Ph. D

Dr. Junfeng Wang, director of the magnetic resonance division and the director of the biomolecular NMR program. He received his PhD degree from National High Magnetic Field Laboratory/Florida State University in 2001. Before joining High Magnetic Field Laboratory as a “Hundred Talents Program” researcher of Chinese Academy of Sciences, he conducted postdoctoral researches at University of Georgia and Harvard Medical School.



Many important biological functions, such as the cross-membrane nutrients transport, neural signal transduction, cell-cell interactions are implemented by membrane proteins, which count for 30% of the protein genomes. Mutations and dysfunctions of membrane proteins and membrane-associated proteins result in various diseases, and over 60% of current drug targets are membrane proteins.  The ongoing researches in the lab focus on membrane protein structure determination and protein-lipid interactions, with the goal of providing structural and dynamical insights on cross-membrane signaling and other protein-membrane recognition events. The primary tool we are using is solution NMR spectroscopy, which is ideal for determining protein structure, characterizing protein dynamics and inter-molecular interactions in solution.


1.Guolin Ma#, Ming Wei#, Lian He#, Chongxu Liu#, Bo Wu, Shenyuan L Zhang, Ji Jing,Xiaowen Liang,Alessandro Senes,Peng Tan,Yunchen Bi,Ling Zhong,Hongjiang Si,Yuequan Shen,Minyong Li,Mi-Sun Lee,Weibin Zhou,Junfeng Wang*,Youjun Wang*,Yubin Zhou*, (2015) Inside-out Ca2+ signalling prompted by STIM1 conformational switch. Nature Comm. 6:7826.
2.Yue Yuan#,Shuchao Ge#,Hongbin Sun#,Xuejiao Dong,Hongxin Zhao,Linna An,Jia Zhang,Junfeng Wang*,Bing Hu*,Gaolin Liang*, (2015), Intracellular Self-Assembly and Disassembly of 19 F Nanoparticles Confer Respective “Off” and “On” 19 F NMR/MRI Signals for Legumain Activity Detection in Zebrafish. ACS Nano 9(5):5117-5124.
3. Yue Yuan#, Hongbin Sun#, Shuchao Ge#, Mengjing Wang, Hongxin Zhao, Lin Wang, Linna An, Jia Zhang,Huafeng Zhang, Bing Hu*, Junfeng Wang*, Gaolin Liang*. (2015),Controlled Intracellular Self-Assembly and Disassembly of 19F Nanoparticles for MR Imaging of Caspase 3/7 in Zebrafish. ACS Nano, 9(1):761-768.
4. Fazhan Shi, Qi Zhang, Pengfei Wang, Hongbin Sun, Jiarong Wang, Xing Rong, Ming Chen, Chenyong Ju, Friedmann Reinhard, Hongwei Chen, Jorg Wrachtrup, Junfeng Wang, Jiangfeng Du*, Single-protein spin resonance spectroscopy under ambient conditions. Science, 347(6226):1135-8.
5. Zhu.L, J. Yang, H. Li, H. B. Sun, J. S. Liu and J. F. Wang (2015). "Conformational change study of dengue virus NS2B-NS3 protease using F-19 NMR spectroscopy." Biochemical and Biophysical Research Communications, 461(4): 677-680.
6. Wan C1, Wu B2, Song Z3, Zhang J3, Chu H4,  Wang A1, Liu Q1, Shi Y3, Li G4, Wang J5, (2015) Insights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2,Chem Phys Lipids, Feb; 186:61-7.
7. Meng Wang, Xin-Ping Wu, Sujuan Zheng, Li Zhao, Lei Li, Li Shen, Yuxian Gao, Nianhua Xue, Xuefeng Guo, Weixin Huang, Zhehong Gan, Frédéric Blanc, Zhiwu Yu, Xiaokang Ke, Weiping Ding, Xue-Qing Gong*, Clare P. Grey, and Luming Peng*(2015), Identification of different oxygen species in oxide nanostructures with 17O solid-state NMR spectroscopy, Science Advances, DOI: 10.1126/sciadv.1400133.
8. Shunrun Chen, Zhiwu Yu#, Qi Zhang, Junping Wang, Teng Zhang, Junfeng Wang*, (2015), Reducing the interfacial reaction between borosilicate sealant andyttria-stabilized zirconia electrolyte by addition of HfO2, Journal of the Eurooean Ceramic Society. 35, 2427–2431.
9.Jinshuai Chen, Zhiwu Yu#, Peizhi Zhu, Junfeng Wang, Zhehong Gan, Jie Wei, Yinghui Zhao and Shicheng Wei, (2015), Effects of fluorine on the structure of fluorohydroxyapatite: a study by XRD, solid-state NMR and Raman spectroscopy, Journal of Materials Chemistry B , 3, 34–38.
10. Xiaozhu Sun, Christopher Branford-White, Zhiwu Yu, Limin Zhu, (2015)Development of universal pH sensors based on textiles, Journal of Sol-Gel Science And Technology, 74, 641–649.
11. Jiarong Wang, Yan Li, Xiaoming Wang, Wei Chen, Hongbin Sun*, Junfeng Wang*. Lipopolysaccharide induces amyloid formation of antimicrobial peptide HAL-2. Biochimica et Biophysica Acta (BBA) – Biomembranes, 2014; 1838(11):2910-2918.
12. Hua Li, Lei Zhu, Shulin Hou, Jing Yang, Junfeng Wang*, Jinsong Liu*, (2014) An inhibition model of BPTI to unlinked dengue virus NS2B-NS3 protease. FEBS Letters, 2014 Aug 25;588(17):2794-9 (*co-corresponding authors)
13. Shi X#, Bi Y#, Yang W#, Guo X, Jiang Y, Wan C, Li L, Bai Y, Guo J, Wang Y, Chen X, Wu B, Sun H, Liu W, Wang J*, Xu C*. (2013) Ca2+ regulates T cell receptor activation by modulating phospholipid electrostatic property. Nature 493(7430):111-5. (#Co-first authors, *co-corresponding authors)
14. Y. Bi, L. Zhu, H. Li, B. Wu, J. Liu*, J. Wang *, (2012) "Backbone 1H, 13C and 15N resonance assignments of Dengue Virus NS2B-NS3p in complex with aprotinin". Biomolecular NMR Assign(In press,doi: 10.1007/s12104-012-9395-9).
15. J. Wang, R. Pielak, M. McClintock and J. Chou, (2009)"Solution Structure and Functional Analysis of the Influenza B Proton Channel", (featured story) Nature Struct. Mol. Biol. 16(12):1267-1272.



Hongbin Sun hbsun@hmfl.ac.cn

Associate professors

Yujuan Wang yjwang@hmfl.ac.cn
Zhiwu Yu zhiwuyu@hmfl.ac.cn

Assistant researchers

Bo Wu bowu@hmfl.ac.cn
Wenping Mao mao@hmfl.ac.cn
Jiarong Wang jiarongwang@hmfl.ac.cn
Jing Yang yj91@hmfl.ac.cn
Yunyan Li yyli@hmfl.ac.cn

Graduate student

Kun Ma makun@hmfl.ac.cn
Juanjuan Liu liujuan@hmfl.ac.cn
Zhe Zhang kids@mail.ustc.ac.cn
Hongxin Zhao zhx@hmfl.ac.cn
Chongxu Liu chongxu@hmfl.ac.cn
Chun Ye cye927@mail.ustc.edu.cn
Dongbei Li 651117671@qq.com
Shuai Xu qtxsxs@163.com
Liang Ge ahyz108@163.com
Maosen Ruan 1406172513@qq.com



2015-09-24 Report

Dr.Jean-Paul Amoureux(University of lille-1)

Presentation Title:New methods for the detection of 14N nuclei in solid state NMR

When: 9:00 on Sep 24th, 2015

Where:322 CHMFL

Host: Dr. Junfeng Wang


2015-06-03 Report

Dr. Haribabu Arthanari (Harvard Medical School)

Presentation Title: Therapeutic Targeting of Protein-Protein Interactions: Deciphering the protein landscape by NMR Spectroscopy

When: 15:00 on June 3rd, 2015 

Where: 201 CHMFL  

Host: Dr. Junfeng Wang




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